Ear Nose and Throat (ENT) Pathogen PCR Reagent Kit
Description
This ENT pathogen polymerase chain reaction (PCR) reagent kit is intended for qualitative, or semi-quantitative, detection of ENT pathogens and the antibiotic-resistant genes associated with the pathogens. This kit includes twelve (12) pre-formulated PCR reactions along with one (1) positive assay control and one (1) exogenous extraction control (EEC) for monitoring the assay performance. Reagents are available in pre-mixed reactions in 2 mL tubes or pre-plated in 96 and 384 well plates.
This RPP qPCR reagent kit consists of pre-formulated qPCR reactions and standards for monitoring assay performance.
- PCR Reaction Mix
- Positive Control (PC)
- Exogenous Extraction Control (EEC)
Sensitivity: < 10copies/ul
Run Time: Approx 1:00 hr (depending on the instrument)
Instrument: Applied Biosystems QuantStudio, Applied Biosystems 7500 Fast Dx (SDS software version 1.4 or later), Bio-Rad CFX96 or CFX384 (Maestro software version 1.1 or later) real-time PCR platforms.
| Catalog# | Reactions (10µl) | Quantity per Kit | Description |
|---|---|---|---|
| ENT-4-12-192T | 192 | 12x2 | PCR Reagents (750 µL) in tubes (pre-mixed). |
| ENT-4-12-96P | 192 | 24 | Multiplex PCR Reagents pre-plated 96 well breakaway plates. (8 reactions [6 samples] per plate) |
| ENT-4-12-192P | 192 | 12 | Multiplex PCR Reagents pre-plated 384 well HALF (½)plates (16 reactions [14 samples] per plate) |
| ENT-4-12-384P | 192 | 6 | PCR Reagents pre-plated 384 well plates (32 reactions [30 samples] per plate) |
| Bacteria | Fungus | AMR |
|---|---|---|
| Acinetobacter baumannii | Aspergillus spp | Beta Lactamase Resistance (CTX-M-Grp1) |
| Aeromonas spp | Candida albicans | Beta Lactamase Resistance (IMP) |
| Alloiococcus otitis | Candida auris | Beta Lactamase Resistance (VIM) |
| Bacteroides fragilis | Candida glabrata | Carbapenem Resistance (KPC) |
| Citrobacter freundii/ braakii/koseri | Candida parapsilosis | Carbapenem Resistance (NDM) |
| Corynebacterium amycolatum | Candida tropicalis | Carbapenem Resistance (OXA-48) |
| Corynebacterium urealyticum | Vancomycin Resistance (Van A) | |
| Cutibacterium acnes/Propionibacterium acnes | Vancomycin Resistance (Van B) | |
| Enterobacter cloacae | ||
| Enterococcus faecalis | ||
| Enterococcus faecium | ||
| Escherichia coli | ||
| Hemophilus influenzae | ||
| Klebsiella (enterobacter) aerogenes | ||
| Klebsiella oxytoca | ||
| Klebsiella pneumoniae | ||
| Moraxella catarrhalis | ||
| Moraxella lacunata | ||
| Peptostreptococcus spp | ||
| Prevotella bivia | ||
| Pseudomonas aeruginosa | ||
| Serratia marcescens | ||
| Staphylococcus aureus | ||
| Staphylococcus epidermidis | ||
| Staphylococcus haemolyticus | ||
| Staphylococcus lugdunensis | ||
| Staphylococcus saprophyticus | ||
| Stenotrophomonas maltophilia | ||
| Streptococcus agalactiae (Group-B) | ||
| Streptococcus pneumoniae | ||
| Streptococcus pyogenes (Group-A) | ||
| Vibrio spp/Vibrio vulnificus |
