Gastrointestinal Pathogen (GI) PCR Reagent Kit
Description
Gastrointestinal Infection (GI) multiplex PCR assays are intended for the qualitative detection of GI track pathogens. The Scienetix GI multiplex PCR reagent kit contains multiplex PCR assays for the detection of bacterial (8), parasitic (6), viral (6) and internal control. The Scienetix multiplex reaction mixes (GRM-1 to GRM-8) are configured as open system reagents (OSR) for use with any real-time PCR system. Fine-tuned multiplex reactions are available in 2ml tubes or pre-plated in 384 well plates.
The Scienetix GI kit consists of pre-formulated multiplex PCR reactions and standards for monitoring assay performance.
- Reaction Mix in Tubes
- Positive Control (PC)
- Exogenous Extraction and RT Control
Sensitivity: < 10copies/ul
Run Time: Approx 1:00 hr (depending on the instrument)
Instrument: Applied Biosystems QuantStudio, Applied Biosystems 7500 Fast Dx (SDS software version 1.4 or later), Bio-Rad CFX96 or CFX384 (Maestro software version 1.1 or later) real-time PCR platforms.
| Catalog# | Reactions (10µl) | Quantity per Kit | Description |
|---|---|---|---|
| GI-4-06-192T | 192 | 6x2 | Multiplex PCR Reagents pre-mixed tubes |
| GI-4-06-96P | 192 | 12 | Multiplex PCR Reagents pre-plated 96 well break away plates (16 reactions [14 samples] per plate) |
| GI-4-06-192P | 192 | 6 | Multiplex PCR Reagents pre-plated 384 well HALF plates (32 reactions [30 samples] per plate) |
| GI-4-06-384P | 192 | 3 | Multiplex PCR Reagents pre-plated 384 well plates (64 reactions [62 samples] per plate) |
| Virus | Bacteria | Parasite |
|---|---|---|
| Human adenovirus-B C | Campylobacter | Ancylostoma duodenale |
| Human astrovirus | Clostridium difficile | Giardia lamblia |
| Norovirus G1 | Enterohemorrhagic E. coli (EHEC) | Cryptosporidium spp. |
| Norovirus G2 | Enteroinvasive E. coli (EIEC) | Entamoeba histolytica |
| Rotavirus A | Helicobacter pylori | Enterobius vermicularis |
| Rotavirus B | Salmonella | Necator americanus |
| Sapovirus | Shigella | |
| Yersinia enterocolitica |
