Respiratory Pathogen Panel (RPP) PCR Reagents Kit
Description
This respiratory pathogen panel (RPP) polymerase chain reaction (PCR) pathogen panel is intended for qualitatively, or semi-quantitatively, detecting the infectious pathogens causing respiratory tract infection and the antibiotic-resistant genes associated with the pathogens. This Scienetix molecular RPP kit contains qPCR multiplex reaction mixes for detecting twenty-one (21) viral and fourteen (14) bacterial pathogens. The Scienetix kit also includes two (2) assays for detecting antibiotic resistance genes and one (1) amplification control. This RPP kit includes ten (10) validated multiplex reaction mixes (RRM-1 to RRM-10) and all associated qPCR assay controls. The RPP kit detection reagents are manufactured and packaged as an open system reagent (OSR) for use with open system platforms. Examples of open system platforms are the Applied Biosystems QuantStudio (Design & Analysis software version 1.5.1 or later), Applied Biosystems 7500 Fast Dx (SDS software version 1.4 or later), Bio-Rad CFX96 or CFX384 (Maestro software version 1.1 or later) real-time qPCR platforms.
This RPP qPCR reagent kit consists of pre-formulated qPCR reactions and standards for monitoring assay performance.
- Reaction Mix (RRM-1 to 10)
- Positive Control (PC)
- Exogenous Extraction + RT Control (EERTC)
Sensitivity: < 10copies/ul
Run Time: Approx 1:00 hr (depending on the instrument)
Instrument: Applied Biosystems QuantStudio, Applied Biosystems 7500 Fast Dx (SDS software version 1.4 or later), Bio-Rad CFX96 or CFX384 (Maestro software version 1.1 or later) real-time PCR platforms.
| Catalog# | Reactions (10µl) | Quantity per Kit | Description |
|---|---|---|---|
| RPP-4-10-192T | 192 | 10x12 | Multiplex PCR Reagents pre-mixed tubes |
| RPP-4-10-96P | 192 | 24 | Multiplex PCR Reagents pre-plated 96 well break away plates (8 reactions [6] samples] per plate) |
| RPP-4-10-192P | 192 | 12 | Multiplex PCR Reagents pre-plated 384 well HALF plates (16 reactions [14 samples] per plate) |
| RPP-4-10-384P | 192 | 6 | Multiplex PCR Reagents pre-plated 384 well plates (32 reactions [30 samples] per plate) |
| Virus | Bacteria | AMR |
|---|---|---|
| Enterovirus-A71 | Bordetella parapertussis | Methicillin Resistance (MecA) |
| Enterovirus-D68 | Bordetella pertussis & holmesii | Vancomycin Resistance (VanA) |
| Human coronavirus 229E | Chlamydophila pneumoniae | Vancomycin Resistance (VanB) |
| Human coronavirus HKU1 | Haemophilus influenzae | |
| Human coronavirus NL63 | Haemophilus influenzae B | |
| Human coronavirus OC43 | Klebsiella pneumoniae | |
| Human Gama 4 (Epstein-Barr, Mono) | Legionella spp. | |
| Human adenovirus | Moraxella catarrhalis | |
| Human bocavirus | Mycoplasma pneumoniae | |
| Human parechovirus | Salmonella typhi/paratyphi | |
| Human parainfluenza 1 virus | Staphylococcus aureus | |
| Human parainfluenza 2 virus | Streptococcus agalactiae (GBS) | |
| Human parainfluenza 3 virus | Streptococcus pneumoniae | |
| Human parainfluenza 4 virus | Streptococcus pyogenes (GAS) | |
| Influenza A virus H1N1 swl | ||
| Human respiratory syncytial viruses (A/B) | ||
| Human metapneumoviruses | ||
| Human rhinovirus | ||
| Influenza A virus | ||
| Influenza B virus | ||
| SARS-Cov2 |
