Sexually Transmitted Infection (STI) PCR Reagent Kit

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Description

Sexually transmitted Infection (STI) multiplex PCR assays are intended for the qualitative detection of sexually transmitted pathogens. The Scienetix STI multiplex PCR reagent kit contains multiplex PCR assays for the detection of bacterial (15), viral (1), Protozoan (1) and internal control. The Scienetix multiplex reaction mixes (SRM-1 to SRM-7) are configured as open system reagents (OSR) for use with any real-time PCR system. Fine-tuned multiplex reactions are available in 2ml tubes or pre-plated 384 well plates.

    KIT CONTENTS

    The Scienetix STI kit consists of pre-formulated multiplex PCR reactions and standards for monitoring assay performance.

    • Reaction Mix (SRM-1 to SRM-6)
    • Positive Control (PC)
    • Exogenous Extraction Control (EEC)

    TECHNICAL SPECIFICATION

    Sensitivity: < 10copies/ul
    Run Time: Approx 1:00 hr (depending on the instrument)
    Instrument: Applied Biosystems QuantStudio, Applied Biosystems 7500 Fast Dx (SDS software version 1.4 or later), Bio-Rad CFX96 or CFX384 (Maestro software version 1.1 or later) real-time PCR platforms.

    ORDERING INFORMATION

    Catalog# Reactions (10µl) Quantity per Kit Description
    STI-4-06-192T 192 6x2 Multiplex PCR Reagents pre-mixed tubes
    STI-4-06-96P 192 12 Multiplex PCR Reagents pre-plated 96 well break away plates (16 reactions [14 samples] per plate)
    STI-4-06-192P 192 6 Multiplex PCR Reagents pre-plated 384 well HALF plates (32 reactions [30 samples] per plate
    STI-4-06-384P 192 3 Multiplex PCR Reagents pre-plated 384 well plates (64 reactions [62 samples] per plate)

    KIT TARGETS

    Bacteria Protozoa Virus
    Chlamydia trachomatis Trichomonas vaginalis Herpes simplex virus 1 anand 2
    Gardnerella vaginalis Herpes simplex virus 2
    Neisseria gonorrhoeae
    Ureaplasma urealyticum/parvum
    Treponema pallidum
    Mycoplasma genitalium
    Haemophilus ducreyi
    Atopobium vaginae
    Megasphaera type II
    Bacterial Vaginosis-Associated Bacterium 1
    Bacterial Vaginosis-Associated Bacterium 2
    Bacterial Vaginosis-Associated Bacterium 3
    Mobiluncus curtisii
    Mobiluncus mulieris
    Megasphaera type I
    Lactobacillus spp.