UTI-48 (Bacteria + Fungus + Antibiotics Markers)
Description
Urinary Tract Infection (UTI) multiplex PCR assays are intended for the qualitative detection of uropathogens and associated Antimicrobial Resistance (AMR) markers. The Scienetix UTI multiplex PCR reagent kit contains multiplex PCR assays for the detection of bacterial (22) and fungal (6) uropathogens, along with associated AMR (18) markers and internal control (1). The Scienetix UTI reaction mixes (URM-1 to URM-16) are configured as open system reagents (OSR) for use with any real-time PCR system. Fine-tuned multiplex reactions are available in 2ml tubes or pre-plated in 384 well plates.
The Scienetix UTI kit consists of pre-formulated multiplex PCR reactions and standards for monitoring assay performance.
- Reaction Mix (URM-1 to URM-8, AMR-1 to AMR-4)
- Positive Control (PC)
- Exogenous Extraction Control (EEC)
Sensitivity: < 10copies/ul
Run Time: Approx 1:00 hr (depending on the instrument)
Instrument: Applied Biosystems QuantStudio, Applied Biosystems 7500 Fast (SDS software version 1.4 or later), Bio-Rad CFX96 or CFX384 (Maestro software version 1.1 or later).
| Catalog# | Reactions (10µl) | Quantity per Kit | Description |
|---|---|---|---|
| UTI-4-12-192T | 192 | 12x2 | PCR Reagents (750 µL) in tubes (pre-mixed) |
| UTI-4-12-96P | 192 | 24 | PCR Reagents pre-plated 96 well plates (pre-plated) (8 reactions [6 samples] per plate) |
| UTI-4-12-192P | 192 | 12 | Reagents pre-plated 96 well plates (pre-plated) (16 reactions [14 samples] per plate) |
| UTI-4-12-384P | 192 | 6 | UTI+AMR Reagents pre-plated 96 well plates (pre-plated) (32 reactions [30 samples] per plate) |
| Bacteria | Fungus | AMR |
|---|---|---|
| Acinetobacter baumannii | Candida albicans | Beta Lactamase (CTX-M-Grp1) |
| Actinotignum schaalii | Candida auris | Beta Lactamase Resistance (SHV) |
| Aerococcus urinae | Candida glabrata | Beta Lactamase Resistance (TEM) |
| Bacteroides fragilis | Candida parapsilosis | Carbapenem Resistance (NDM) |
| Citrobacter freundii/braakii/koseri | Candida tropicalis | Carbapenem Resistance (OXA) |
| Enterobacter cloacae | Epidermophyton floccosum | Fluoroquinolone Resistance (qnr) |
| Enterococcus faecalis | Trichophyton rubrum | Fluoroquinolone Resistance (gyrA) |
| Enterococcus faecium | Methicillin Resistance (MecA) | |
| Escherichia coli | Sulfonamide Resistance (Sul1) | |
| Klebsiella aerogenes | Tetracycline Resistance (tetB) | |
| Klebsiella oxytoca | Tetracycline Resistance (tetM) | |
| Klebsiella pneumoniae | Trimethoprim Resistance (DfrA1) | |
| Morganella morganii | Vancomycin Resistance (VanA) | |
| Prevotella bivia | Vancomycin Resistance (VanB) | |
| Proteus mirabilis | Vancomycin Resistance (VanM) | |
| Proteus vulgaris | ||
| Pseudomonas aeruginosa | ||
| Serratia marcescens | ||
| Staphylococcus aureus | ||
| Staphylococcus haemolyticus | ||
| Staphylococcus saprophyticus | ||
| Streptococcus agalactiae | ||
| Streptococcus pyogenes |
